protein loading controls (Santa Cruz Biotechnology)
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protein loading controls
Protein Loading Controls, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 21541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+loading+control/GAPDH+Antibody/pm41731023-84-25-21
Average 97 stars, based on 21541 article reviews
Protein Loading Controls, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 21541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+loading+control/GAPDH+Antibody/pm41731023-84-25-21
Average 97 stars, based on 21541 article reviews
protein loading controls - by Bioz Stars,
2026-09
97/100 stars
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Incubation:Article Title: Unraveling chain specific ubiquitination in cells using tandem ubiquitin binding entities Article Snippet: Blots were washed 3 times with 1X PBS-T followed by incubation with HRP-conjugated secondary antibody (1:1000) for 60 min. After washing the blots 3 times with 1X PBS-T, the blots were developed with enhanced chemiluminescence substrates and images were acquired with Odyssey ® Fc Imager (LI-COR) and processed using LI-COR Image Studio (version 5.2.5). .. Blots were stripped and incubated for 60 min with an HRP-conjugated anti-GAPDH antibody for obtaining Article Title: Unraveling chain specific ubiquitination in cells using tandem ubiquitin binding entities. Article Snippet: Blots were washed 3 times with 1X PBS-T followed by incubation with HRP-conjugated secondary antibody (1:1000) for 60 min. After washing the blots 3 times with 1X PBS-T, the blots were developed with enhanced chemiluminescence substrates and images were acquired with Odyssey® Fc Imager (LI-COR) and processed using LI-COR Image Studio (version 5.2.5). .. Blots were stripped and incubated for 60 min with an HRP-conjugated anti-GAPDH antibody for obtaining Article Title: Kinome profiling in pediatric brain tumors as a new approach for target discovery. Article Snippet: Progression in pediatric brain tumor growth is thought to be the net result of signaling through various protein kinasemediated networks driving cell proliferation.. Defining new targets for treatment of human malignancies, without a priori knowledge on aberrant cell signaling activity, remains exceedingly complicated.. Here, we introduce kinome profiling using flow-through peptide microarrays as a new concept for target discovery. Article Title: PTK787/ZK 222584 inhibits tumor growth promoting mesenchymal stem cells: kinase activity profiling as powerful tool in functional studies. Article Snippet: Laemmli sample buffer was added and the lysate was boiled for 5 min. Proteins were separated using a 10% SDS/polyacrylamide gel, blotted onto a PVDF membrane (Millipore, Etten-Leur, Netherlands), blocked with Tris-buffered saline + 1% Tween-20 and 5% Bovine Serum Albumine (Sigma Aldrich, Zwijndrecht, Netherlands) (TBST/ BSA). .. Blots were incubated overnight at RT in TBST/BSA containing a primary antibody anti-FAK, anti-pFAK(Tyr397) (BD Biosciences, USA), anti-ERK, anti-pERK(Thr202/Tyr204), antiCDC2, anti-pCDC2(Tyr15) (Cell Signaling, USA) (all 1:2,000), followed by incubation with the appropriate secondary peroxidaseconjugated antibodies (Dako, Denmark) (1:5,000) at RT for 1 h. Antibody binding was visualized by enhanced chemoluminescence. β-actin was probed as a Control:Article Title: Unraveling chain specific ubiquitination in cells using tandem ubiquitin binding entities Article Snippet: Blots were washed 3 times with 1X PBS-T followed by incubation with HRP-conjugated secondary antibody (1:1000) for 60 min. After washing the blots 3 times with 1X PBS-T, the blots were developed with enhanced chemiluminescence substrates and images were acquired with Odyssey ® Fc Imager (LI-COR) and processed using LI-COR Image Studio (version 5.2.5). .. Blots were stripped and incubated for 60 min with an HRP-conjugated anti-GAPDH antibody for obtaining Article Title: CRISPR/Cas9-mediated correction of mutated copper transporter ATP7B. Article Snippet: Samples were blotted onto PVDF membranes and blocked with 5% semiskimmed milk powder (Roth, Karlsruhe, Germany) in Tris-buffered saline supplemented with 0.1% Tween 20 (Merck, Darmstadt, Germany). .. For detection of ATP7B protein (ATPase7), a monoclonal rabbit anti-human ATP7B antibody (1:1,000, ab124973, Abcam, Cambridge, UK) was added overnight. β-Actin was assessed for Article Title: Monocarboxylate transporter functions and neuroprotective effects of valproic acid in experimental models of amyotrophic lateral sclerosis. Article Snippet: Protein concentration was determined using a protein assay kit (Bio-Rad, Hercules, CA, USA). .. Then, 25 μg of protein was subjected to SDS/10% PAGE and blotted with primary antibodies (1:1,000 dilution) [MCT1 (SLC16A1) (Cat. No.: AMT011, Alomone Labs, Israel) and SMCT1 (SLC5A8/AIT) (Cat. No.: ab99064, Abcam, Cambridge, MA, USA)]. β-Actin (ACTB) was used as a Article Title: Unraveling chain specific ubiquitination in cells using tandem ubiquitin binding entities. Article Snippet: Blots were washed 3 times with 1X PBS-T followed by incubation with HRP-conjugated secondary antibody (1:1000) for 60 min. After washing the blots 3 times with 1X PBS-T, the blots were developed with enhanced chemiluminescence substrates and images were acquired with Odyssey® Fc Imager (LI-COR) and processed using LI-COR Image Studio (version 5.2.5). .. Blots were stripped and incubated for 60 min with an HRP-conjugated anti-GAPDH antibody for obtaining Article Title: TRIB2 supports a high glycolytic phenotype in melanoma cells Article Snippet: Secondary antibodies were conjugated with horseradish peroxidase (GE Healthcare Life Sciences) and visualized by the ECL detection solution. .. Whenever possible based on the molecular size of the proteins, membranes were re-stained with different antibodies, including antibodies for Article Title: Pharmacological Inhibition of cFLIP Targets Breast Cancer Stem Cells. Article Snippet: Total cellular proteins were extracted from cultured cells and 30 μg analysed by Western blotting as described28. cFLIP antibodies used were purchased from Santa Cruz (5D8, sc136160) and Enzo Life Sciences (7F10, ALX-804961-0100). .. To quantitate Western data, the pixel intensity of each band was quantified relative to its Article Title: Kinome profiling in pediatric brain tumors as a new approach for target discovery. Article Snippet: Progression in pediatric brain tumor growth is thought to be the net result of signaling through various protein kinasemediated networks driving cell proliferation.. Defining new targets for treatment of human malignancies, without a priori knowledge on aberrant cell signaling activity, remains exceedingly complicated.. Here, we introduce kinome profiling using flow-through peptide microarrays as a new concept for target discovery. Article Title: PTK787/ZK 222584 inhibits tumor growth promoting mesenchymal stem cells: kinase activity profiling as powerful tool in functional studies. Article Snippet: Laemmli sample buffer was added and the lysate was boiled for 5 min. Proteins were separated using a 10% SDS/polyacrylamide gel, blotted onto a PVDF membrane (Millipore, Etten-Leur, Netherlands), blocked with Tris-buffered saline + 1% Tween-20 and 5% Bovine Serum Albumine (Sigma Aldrich, Zwijndrecht, Netherlands) (TBST/ BSA). .. Blots were incubated overnight at RT in TBST/BSA containing a primary antibody anti-FAK, anti-pFAK(Tyr397) (BD Biosciences, USA), anti-ERK, anti-pERK(Thr202/Tyr204), antiCDC2, anti-pCDC2(Tyr15) (Cell Signaling, USA) (all 1:2,000), followed by incubation with the appropriate secondary peroxidaseconjugated antibodies (Dako, Denmark) (1:5,000) at RT for 1 h. Antibody binding was visualized by enhanced chemoluminescence. β-actin was probed as a Polyacrylamide Gel Electrophoresis:Article Title: Monocarboxylate transporter functions and neuroprotective effects of valproic acid in experimental models of amyotrophic lateral sclerosis. Article Snippet: Protein concentration was determined using a protein assay kit (Bio-Rad, Hercules, CA, USA). .. Then, 25 μg of protein was subjected to SDS/10% PAGE and blotted with primary antibodies (1:1,000 dilution) [MCT1 (SLC16A1) (Cat. No.: AMT011, Alomone Labs, Israel) and SMCT1 (SLC5A8/AIT) (Cat. No.: ab99064, Abcam, Cambridge, MA, USA)]. β-Actin (ACTB) was used as a Membrane:Article Title: Monocarboxylate transporter functions and neuroprotective effects of valproic acid in experimental models of amyotrophic lateral sclerosis. Article Snippet: Protein concentration was determined using a protein assay kit (Bio-Rad, Hercules, CA, USA). .. Then, 25 μg of protein was subjected to SDS/10% PAGE and blotted with primary antibodies (1:1,000 dilution) [MCT1 (SLC16A1) (Cat. No.: AMT011, Alomone Labs, Israel) and SMCT1 (SLC5A8/AIT) (Cat. No.: ab99064, Abcam, Cambridge, MA, USA)]. β-Actin (ACTB) was used as a Western Blot:Article Title: Pharmacological Inhibition of cFLIP Targets Breast Cancer Stem Cells. Article Snippet: Total cellular proteins were extracted from cultured cells and 30 μg analysed by Western blotting as described28. cFLIP antibodies used were purchased from Santa Cruz (5D8, sc136160) and Enzo Life Sciences (7F10, ALX-804961-0100). .. To quantitate Western data, the pixel intensity of each band was quantified relative to its Binding Assay:Article Title: Kinome profiling in pediatric brain tumors as a new approach for target discovery. Article Snippet: Progression in pediatric brain tumor growth is thought to be the net result of signaling through various protein kinasemediated networks driving cell proliferation.. Defining new targets for treatment of human malignancies, without a priori knowledge on aberrant cell signaling activity, remains exceedingly complicated.. Here, we introduce kinome profiling using flow-through peptide microarrays as a new concept for target discovery. Article Title: PTK787/ZK 222584 inhibits tumor growth promoting mesenchymal stem cells: kinase activity profiling as powerful tool in functional studies. Article Snippet: Laemmli sample buffer was added and the lysate was boiled for 5 min. Proteins were separated using a 10% SDS/polyacrylamide gel, blotted onto a PVDF membrane (Millipore, Etten-Leur, Netherlands), blocked with Tris-buffered saline + 1% Tween-20 and 5% Bovine Serum Albumine (Sigma Aldrich, Zwijndrecht, Netherlands) (TBST/ BSA). .. Blots were incubated overnight at RT in TBST/BSA containing a primary antibody anti-FAK, anti-pFAK(Tyr397) (BD Biosciences, USA), anti-ERK, anti-pERK(Thr202/Tyr204), antiCDC2, anti-pCDC2(Tyr15) (Cell Signaling, USA) (all 1:2,000), followed by incubation with the appropriate secondary peroxidaseconjugated antibodies (Dako, Denmark) (1:5,000) at RT for 1 h. Antibody binding was visualized by enhanced chemoluminescence. β-actin was probed as a |